25.8.20
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Queen's University Short Course in Environmental DNA

Taylor Swanburg

Environmental DNA is increasingly used in everything from disease (e.g. COVID-19) and algal bloom surveillance, species-at-risk management, invasive species detection, and assessment of environmental health and is being incorporated into standard protocols in each of these domains by various levels of government. This short course teaches students about environmental DNA applications and tools for environmental assessment and biodiversity and invasive species monitoring.

Skills / Knowledge

  • Define environmental DNA and understand its sources in nature.
  • Understand the fate and duration of eDNA in natural environments.
  • Undertake basic lab methods including DNA isolation, agarose electrophoresis, DNA quantification, and polymerase chain reaction (PCR).
  • Evaluate the outputs of distinct methods for assaying eDNA: quantitative PCR, digital droplet PCR, and DNA metabarcoding.
  • Analyze data (e.g.FASTQ files) from Illumina sequence from metabarcoding libraries.
  • Understand environmental DNA sampling protocols.
  • Know basic set-up for qualitative and digital droplet PCR experiments.

Issued on

July 29, 2022

Expires on

Does not expire